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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been noticed in lots of tumors, which leads to chromosome instability, and so may well lead to tumorigenesis. This protein was uncovered to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a crucial part in advertising cell-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, happen to be described. Two added splice variants had been documented but in depth nucleotide sequence data is just not yet accessible.
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Blocking peptides are peptides that bind especially on the target antibody and block antibody binding. These peptide ordinarily contains the epitope recognized by the antibody.
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This guide outlines additional attainable optimization of mobile and immunoassay parameters to guarantee the very best effects are...
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This guideline outlines additional possible optimization of cellular and immunoassay parameters to be sure the very best final results are...
Antibodies certain to the blocking peptide no longer bind on the epitope over the target protein. By evaluating the staining through the blocked antibody compared to the antibody on your own, you can see which staining is precise.
Goal Data Cyclin E belongs to your remarkably conserved cyclin household, whose associates are characterised by a extraordinary periodicity in protein abundance through the cell cycle. Cyclins operate as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which contribute towards the temporal coordination of each mitotic occasion. Cyclin E types a complex with and functions as a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S changeover. This protein accumulates for the G1-S section boundary which is degraded as cells progress by means of S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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